11 year papers

Biotechnology and its Applications CBSE Class 12 PYQs

Biotechnology and its Applications CBSE Class 12 PYQs: all 23 questions from 50 official papers (2016–2026), by concept. Asked in 10 of 10 years.

Updated2 min read
Full name
CBSE Class 12 Board Examination
Conducted by
Central Board of Secondary Education
Frequency
Annual board exams (as notified)
Sections
Subject-wise theory papers (as opted)
Official site
cbse.gov.in ↗

Explore: All CBSE Class 12 chapters · CBSE Class 12 Biology PYQs · CBSE Class 12 hub · Syllabus & pattern · Class 12 2026

Answer first

Biotechnology and its Applications PYQs (previous year questions) for CBSE Class 12: Biotechnology and its Applications (Biology) had 23 questions across the 50 official CBSE Class 12 papers we tagged (2016–2026), and appeared in 10 of them. Asked in 10 of 10 years. The concepts asked in the most papers: Gene Therapy, Bt Crops, RNA Interference.

Year-wise question count

Asked in 10 of 10 years.

Most-asked concepts

Sorted by the number of papers a concept appeared in.

ConceptQuestionsPapersYears
Gene Therapy442025, 2023, 2019, 2016
Bt Crops332026, 2022, 2017
Genetically Engineered Insulin332020, 2018, 2017
RNA Interference332025, 2020, 2018
Transgenic Animals322026, 2024
Biopiracy222023, 2016
Biosafety222025, 2024
GM Crops222024, 2019
Stem Cells112023

How to practise Biotechnology and its Applications PYQs

  1. Start with the concepts at the top of the table. They have come back the most.
  2. Solve one year’s Biotechnology and its Applications questions from the official PDF against the clock, then check the answer listed below.
  3. Log every miss as a concept gap, a calculation slip or a misread. Each needs a different fix.
  4. Once a concept is clean in two papers, move to the next. Then sit a full paper from the year pages.

Questions by year

Each line gives the question number and paper, type, concept, our one-line summary, with a link to the official PDF.

CBSE Class 122026

  • Q.14 (Biology (57/1/1)) · Assertion-Reason · Transgenic Animals: Nutritional value of milk from transgenic cow Rosie PDF
  • Q.27 (Biology (57/1/1)) · Short answer · Bt Crops: Cry gene in Bt spores, its effect on pests and how Bt corn resists PDF

CBSE Class 122025

  • Q.2 (Biology (57/1/1)) · MCQ · Biosafety: Full form of GEAC PDF
  • Q.12 (Biology (57/1/1)) · MCQ · RNA Interference: How RNAi makes tobacco resistant to nematodes PDF
  • Q.21 (Biology (57/1/1)) · Very short answer · Gene Therapy: Why ADA patients need periodic lymphocyte infusion and a permanent cure PDF

CBSE Class 122024

  • Q.9 (Biology (57/1/1)) · MCQ · Transgenic Animals: Transgenic animal used to test polio vaccine safety PDF
  • Q.11 (Biology (57/1/1)) · MCQ · GM Crops: Improved trait of Golden rice PDF
  • Q.16 (Biology (57/1/1)) · Assertion-Reason · Biosafety: Role of GEAC and ecological effects of GM organisms PDF
  • Q.27 (Biology (57/1/1)) · Short answer · Transgenic Animals: Define transgenic animals; first transgenic cow and its value PDF

CBSE Class 122023

  • Q.12 (Biology (57/1/1)) · MCQ · Biopiracy: Who challenged the turmeric wound-healing patent PDF
  • Q.13 (Biology (57/1/1)) · Assertion-Reason · Gene Therapy: Periodic lymphocyte infusion in ADA gene therapy and lymphocyte lifespan PDF
  • Q.24 (Biology (57/1/1)) · Short answer · Stem Cells: Features, sources and medical uses of stem cells PDF

CBSE Class 122022

  • Q.13 (Biology Term 2 (57/1/1)) · Case-based · Bt Crops: Interpret leaf damage by three corn borers on Bt and non-Bt corn PDF

CBSE Class 122020

  • Q.5 (Biology (57/1/1)) · MCQ · RNA Interference: Vector used to put nematode-specific genes into tobacco PDF
  • Q.22 (Biology (57/1/1)) · Case-based · Genetically Engineered Insulin: Proinsulin processing, Eli Lilly technique and chain linkage PDF

CBSE Class 122019

  • Q.11 (Biology (57/1/1)) · Short answer · GM Crops: Four ways GMOs have raised crop output PDF
  • Q.22 (Biology (57/1/1)) · Short answer · Gene Therapy: Two ADA-deficiency treatments: repeated enzyme therapy vs permanent cure PDF

CBSE Class 122018

  • Q.5 (Biology (57/1)) · Very short answer · Genetically Engineered Insulin: Chemical change that turns proinsulin into mature insulin PDF
  • Q.22 (Biology (57/1)) · Short answer · RNA Interference: How Agrobacterium vectors helped control nematode infestation in tobacco PDF

CBSE Class 122017

  • Q.4 (Biology (57/1)) · Very short answer · Bt Crops: What Cry genes are and which organism carries them PDF
  • Q.20 (Biology (57/1)) · Short answer · Genetically Engineered Insulin: Steps in producing artificial insulin PDF

CBSE Class 122016

  • Q.3 (Biology (57/1)) · Very short answer · Biopiracy: Define biopiracy PDF
  • Q.20 (Biology (57/1)) · Short answer · Gene Therapy: Enzyme replacement therapy for ADA deficiency and its drawbacks PDF

Other Biology chapters

FAQ

How many Biotechnology and its Applications questions come in CBSE Class 12?

In the official papers we tagged: 2 in 2026, 3 in 2025, 4 in 2024, 3 in 2023, 1 in 2022, 2 in 2020, 2 in 2019, 2 in 2018, 2 in 2017 and 2 in 2016. That is 23 questions in all, about 8% of Biology.

Which Biotechnology and its Applications topics repeat in CBSE Class 12?

Asked in the most papers: Gene Therapy, Bt Crops, RNA Interference. The concept table lists every one with its count.

Are these official CBSE Class 12 questions?

Yes. Every question is from an official CBSE Class 12 paper hosted on our year pages. The one-line summaries are ours; open the PDF linked beside each question for the full text.